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KMID : 0358419730160010013
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Korean Journal of Obstetrics and Gynecology
1973 Volume.16 No. 1 p.13 ~ p.23
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Immunological Determination of Luteinizing Hormone in Urine during Normal Menstrual Cycle
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김용성/Kim YS
신면우/Shin MW
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Abstract
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For the immunologic determination of Luteinizing Hormone (L.H.) in urine during normal menstrual cycle, basic experiments and quantitative assay has been performed using hemagglutination inhibition reaction based upon the cross reaction between the H.C.G.-antiserum to L.H.. Following results were obtained. 1. Human Chorionic Gonadotropin(H.C.G.) supplied by Mann Research Laboratories, New York, N.Y. have been used as antigen for the immunization of rabbits and relative high titer of antiserum was obtained through the effects of Freund`s adjuvant and booster injections. 2. Gel diffusion method of Ouchterlony revealed only one clear precipitation line between the H.C.G. and its antiserum, indicating that the used antigen was relatively pure and the antisera contained negligible amount of no-specific antibody. 3. The sensitivity of hemagglutination inhibition reaction in this study was about 100-150 I.U./L.. 4. Early morning samples of urine were collected daily or every other day from 5 menstrual cycle of 4 normally menstruating women and basal body temperatures were recorded daily for the estimation of the date of ovulation. 5. One volume of urine was treated with 4 volumes of acetone and L.H. containing fraction was washed with one volume of absolute alcohol and ether, then dissolved in 1/10 volume of pH 6.4 phosphate buffered saline, thus concentrating the original specimen 10 times. 6. Quantitative determination of L.H. with serial 1.58-fold dilution of the urine concentrates revealed a single preovulatory peak (300-400 I.U./L.) and relative elevated levels were found several days before and after each peak and the lowest levels of L.H. were found before and after each menses.
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